Assoc. Prof. Dr. Çağdaş D. Son

Office Room: 227

Tel: +90 312 210 6472

Fax: +90 312 210 7976

E - Mail: cson[-at-]metu.edu.tr

Web: http://www.metu.edu.tr/~cson

Bachelor of Science
[09/1993 to 06/1997] Middle East Technical University, Ankara, Turkey
[09/1993 to 06/1997] Middle East Technical University, Ankara, Turkey
•Organic Chemistry
Master of Science
[09/1997 to 06/1999] Middle East Technical University, Ankara, Turkey
[08/1999 - 12/2004] University of Tennessee, Knoxville, TN
Post Doc.
[01/2005 - 05/2006] University of Tennessee, Knoxville, TN
•Receptor ligand interactions (GPCRs)
Post Doc.
[06/2006 - 01/2009] California Institute of Technology
•Nicotinic Acetylcholine Receptors, Confocal Microscopy

Research Interest: Membrane proteins, Protein-protein interactions, GPCRs, nAChRs, Fluorescence proteins, Confocal microscopy, Total Internal Reflection Fluorescence microscopy, FRET, HPLC, Mass spectrometry, MALDI-TOF, protein purification, Photo affinity cross-linking

Description of research activities: Currently our research focused on the identification of protein-protein interactions by quantitative Förster resonance energy transfer (FRET) measurements as well as by florescence intensity ratio (FIR) analysis in live cells. We are interested in G-protein Coupled Receptor (GPCR) dimerization, and effects of post-translational modifications and ligand interactions on receptor dimerization.

Selected Publications:

1.Srinivasan R, Pantoja R, Moss FJ, Mackey ED, Son CD, Miwa J, Lester HA. Nicotine up-regulates {alpha}4{beta}2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. J Gen Physiol (2011) Jan;137(1):59-79

2.Cagdas D. Son, Fraser J. Moss, Bruce N. Cohen, Henry A. Lester.
Nicotine Normalizes Intracellular Subunit Stoichiometry of Nicotinic Receptors Carrying Mutations linked to Autosomal Dominant Nocturnal Frontal Lobe Epilepsy. Molecular Pharmacology (2009) May;75(5):1137-48

3.Lester HA, Xiao C, Srinivasan R, Son CD, Miwa J, Pantoja R, Banghart MR, Dougherty DA, Goate AM, Wang JC. Nicotine is a Selective Pharmacological Chaperone of Acetylcholine Receptor Number and Stoichiometry. Implications for Drug Discovery. AAPS J. (2009) March; 11(1):167-77.

4.George K. E. Umanah, Cagdas Son, FaXiang Ding, Fred Naider and Jeffrey M. Becker. Cross-Linking of a DOPA-Containing Peptide Ligand into Its G Protein-Coupled Receptor. Biochemistry (2009) March; 48(9):2033-44.

5.Li-Yin Huang, George Umanah, Melinda Hauser, Cagdas Son, Boris Arshava, Fred Naider, and Jeffrey M. Becker. Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment. Biochemistry (2008) May; 47(20):5638-48.

6.Byung-Kwon Lee, Kyung-Sik Jung, Cagdas Son, Heejung Kim, Nathan C. VerBerkmoes, Boris Arshava, Fred Naider, and Jeffrey M. Becker. Affinity purification and Characterization of a G-Protein Coupled Receptor, Saccharomyces cerevisiae Ste2p. Protein Expression and Purification (2007) Nov; 56(1):62-71.

7.Cagdas D. Son, Hasmik Sargsyan, Hurst GB, Fred Naider, Jeffrey M. Becker. Analysis of Ligand-Receptor Cross-linked Fragments by Mass Spectrometry. Journal of Peptide Research (2005) Mar; 65(3):418-26

8.Cagdas D. Son, Hasmik Sargsyan, Fred Naider, Jeffrey M. Becker. Identification of binding regions of the Saccharomyces cerevisiae alpha-factor pheromone receptor (Ste2p) by photo-affinity cross-linking. Biochemistry (2004) Oct 19; 43(41):13193-203

9.Andjelka Ćelić, Negin P. Martin, Cagdas D. Son, Jeffrey M. Becker, Fred Naider, and Mark E. Dumont. Sequences in the Intracellular Loops of the Yeast Pheromone Receptor Ste2p Required for G Protein Activation. Biochemistry (2003) Mar 18; 42(10):3004-17.

10.Lee BK, Lee YH, Hauser M, Son CD, Khare S, Naider F, Becker JM. Tyr266 in the sixth transmembrane domain of the yeast alpha-factor receptor plays key roles in receptor activation and ligand specificity. Biochemistry (2002) Nov 19; 41(46):13681-9.

11.L. Keith Henry, Sanjay Khare, Cagdas Son, Fred Naider and Jeffrey M. Becker. Identification of a contact region between the tridecapeptide alpha-factor mating pheromone of Saccharomyces cerevisiae and its G protein-coupled receptor by photo-affinity labeling. Biochemistry (May 2002) 14; 41(19):6128-39.

Graduate program affiliation: Biology, Biochemistry, Molecular Biology and Genetics, Biotechnology

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