FACULTY OF ARTS AND SCIENCESDEPARTMENT OF BIOLOGICAL SCIENCES: BIOLOGY/MOLECULAR BIOLOGY & GENETICS


Faculty

Prof. Dr. Gülay ÖZCENGİZ

Middle East Technical University, Department of Biological Sciences, 06800 Ankara/TURKEY

Office Room: 225

Tel: +90 312 210 5170

Fax: +90 312 210 7976

E - Mail: ozcengiz[-at-]metu.edu.tr

Web: http://molmicrobio.metu.edu.tr

Education: M.S., Ph.D. METU


Research Interest: Molecular Microbiology and Genetics, Proteomics, Biotechnology


Description of research activities:
Proteomics of Bacillus subtilis to further analyse the role of dipeptide antibiotic biosynthesis in quorum sensing.
Proteome dynamics of heavy metal stress response in Phanerochaete chrysosporium.
Proteomics and immunoproteomics of Bordetella pertussis
Development of DNA and subunit vaccines against pasteurollosis.
Pathway engineering to improve clavulinic acid and cephamycin C biosynthesis in Streptomyces clavuligerus.
Transcriptional regulation of cephamycin C biosynthetic gene cluster.


Selected Publications:

Yılmaz Ç., Özcengiz G. 2017. Antibiotics: pharmacokinetics, toxicity, resistance and multidrug efflux pumps. Biochemical Pharmacology. (In press) http://dx.doi.org/10.1016/j.bcp.2016.10.005

Ünsaldı, E., Kurt-Kızıldoğan, A., Voigt, B., Becher, D., Özcengiz, G. 2017. Proteome-wide alterations in an industrial clavulanic acid producing strain of Streptomyces clavuligerus. Synthetic and Systems Biotechnology, 2: 39-48.

Yılmaz, Ç., Apak, A., Özcengiz, E., Özcengiz, G. 2016. Immunogenicity and protective efficacy of recombinant iron superoxide dismutase protein from Bordetella pertussis in mice models. Microbiology and Immunology, 60(11): 717-724.

Özcengiz, G., Öğülür, İ. 2015. Biochemistry, genetics and regulation of bacilysin biosynthesis and its significance more than an antibiotic. New biotechnology, 32(6): 612-619.

Barredo, J. L., Ozcengiz, G., Demain, A. L. 2014. Advances in Beta-Lactam Antibiotics. In Antimicrobial Compounds (pp. 115-147). Springer Berlin Heidelberg.


Özcengiz G., Demain A. L. 2013. Recent advances in the biosynthesis of penicillins, cephalosporins and clavams and its regulation. Biotechnology Advances. 31: 287-311

Kurt A., Alvarez-Alvarez R., Liras P., Özcengiz G. 2013. Role of cmcH-ccar intergenic region and ccar overexpression in cephamycin C biosynthesis in Streptomyces clavuligerus. Applied Microbiology and Biotechnology, 97(13): 5869-80.

Okay, S., Özcengiz, E. and Özcengiz G. 2012. Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice. Research in Veterinary Science. 93: 1261 - 1265

Köroğlu, T.E., Öğülür, İ., Mutlu, S., Yazgan-Karataş, A. and Özcengiz, G. 2011. Global Regulatory Systems Operating in Bacilysin Biosynthesis in Bacillus subtilis.Journal of Molecular Microbiology and Biotechnology. 20:144-155.

Tefon, B.E., Kühnel, S., Özcengiz, E., Becher, D., Hecker, M. andÖzcengiz, G. 2011. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine. 29: 3583-3595.

Yıldırım V., Özcan, S., Becher, D., Buettner, K., Hecker, M., and Özcengiz, G. 2011. Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure. BMC Proteome Science.9: 12-27.

Özcan, O., İçgen, B. and Özcengiz, G. 2010. Pretreatment of poultry litter improves Bacillus thuringiensis-based biopesticides production. Bioresource Technology. 101 (7): 2401-2404

Özcengiz, G., Okay, S., Ünsaldı, E., Taşkın, B., Liras, P., Piret, J. 2010. Homologous expression of aspartokinase (ask) gene in Streptomyces clavuligerus and its hom-deleted mutant:Effects on
cephamycin C production. Bioengineered Bugs. 1 (3): 1-7.

Altındis, E., Tefon, B.E, Yıldırım, V. Özcengiz, E., Becher, D., Hecker, M. and Özcengiz, G. 2009. Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins. Vaccine. 27: 542-548.

Yılmaz, E. İ., Çaydaşı, A. K. and Özcengiz, G. 2008. Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus. Journal of Industrial Microbiology and Biotechnology. 35: 1-7.

Okay, S. Tefon, B., Özkan, M. and Özcengiz, G. 2008. Expression of chitinase A (chiA) gene from a local isolate of Serratia marcescens in Coleoptera-specific Bacillus thuringiensis. Journal of Applied Microbiology. 104: 161-170.

Özcan, S., Yıldırım V., Kaya, L., Albrecht, Dick, Becher, D., Hecker, M., and Özcengiz, G. 2007. Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal response. Proteomics. 2007. 7:1249-1260.

Tokcaer, Z., Bayraktar, E., Mehmetoğlu, Ü., Özcengiz, G and Alaeddinoğlu, N.G. 2006. Response surface optimization of antidipteran delta-endotoxin production by Bacillus thuringiensis subsp. israelensis HD 500. Process Biochemistry. 41: 350–355.

Kurt, A., Özkan, M. and Özcengiz, G. 2005. Inorganic phosphate has a crucial effect on Cry3Aa delta-endotoxin production. Letters in Applied Microbiology. 41: 303-308.

Özkan, M., Yılmaz, E. İ., Lynd, L. R. and Özcengiz, G. 2004. Cloning and expression of Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization. Canadian Journal of Microbiology. 50: 845-851.

Tunca, S., Yılmaz, E. I., Piret, J., Liras, P. and Özcengiz, G. 2004. Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerus. Research in Microbiology. 155: 525-534.

Tüzün, I., Karataş A. Y., Şeşenoğlu, Ö., Demir, A. S. and Özcengiz, G. 2003. 2-Amino-N-(2-furylmethyl)propanamide as a novel alanylglycine equivalent synthesized by bacilysin synthetase. Enzyme and Microbial Technology. 33: 725-728.

Karataş, A., Çetin, S. and Özcengiz, G. 2003. The effects of insertional mutations in comQ, srfa, spoOH, spoOA and abrB genes on bacilysin biosynthesis in Bacillus subtilis. Biochimica et Biophysica Acta-Gene Structure and Expression. 1626 (1-3): 51-56.


Graduate program affiliation: Biology, Biotechnology, Biochemistry