Prof. Dr. Hüseyin Avni ÖKTEM

Middle East Technical University, Department of Biological Sciences, 06800 Ankara/TURKEY

Office Room: 226

Tel: +90 312 210 5172

Fax: +90 312 210 7976

E - Mail: haoktem[-at-]metu.edu.tr

Web: http://plantbiotech.metu.edu.tr/plantbiotech

Education: BSc., 1985, Biology, METU;
Msc. 1987 Biochemistry, METU;
Certificate in Neurochemistry, 1987-1988, Hungarian Academey of Sciences, Biological Research Center;
PhD.1990, Joszef Attila Univ, Szged Hungary

Research Interest: Nanobiotechnology, Nanotechnology, CBRNe, Plant biotechnology, Algal biotechnology, Transgenic technologies, Biosensing technologies, GMO, Signal transduction, Molecular diagnostics, Array technologies, Biosensors, Aptamers, Whole cell biosensors, Renewable bio-energy technologies

Description of research activities: Homeland security technologies, Development of bio-agent detection technologies, New generation CBRNe decontamination technologies, CBRNe applications, Nucleic acid and protein arrays, biological applications of nanoparticles, Genetic manipulation of crop plants and algae, GMO detection technologies, Renewable bio-energy technologies;

Selected Publications:

Mert Donmez, Hüseyin Avni Öktem, M. Deniz Yılmaz, Ratiometric Fluorescence Detection of an Anthrax Biomarker with Eu3+- Chelated Chitosan Biopolymers. Carbohydrate Polymers, 180, 226–230, 2018.

Işkın Kose Engin, Deniz Cekmecelioglu, Ayse Meral Yücel, Hüseyin Avni Öktem. Enhancement of heterotrophic biomass production by Micractinium sp.ME05. Waste and Biomass Valorization, DOI:10.1007/ö12649-017-9846-8, 2017.

Bayraç C., Eyidoğan F., Öktem H.A. DNA aptamer based colorimetric detection platform for Salmonella Enteritidis. Biosensors Bioelectronics, Accepted. DOI http://dx.doi.org/10.1016/j.bios.2017.06.029.

Kayıhan C, Eyidoğan F, Yücel M, Öktem HA. Physiological, biochemical and transcriptomic responses to boron toxicity in leaf and root tissues of contrasting wheat cultivars. Plant Molecular Biology Reporter, 35:97–109. DOI 10.1007/s11105-016-1008-9, 2017.

Bayrac C., Öktem H.A. Evaluation of Staphylococcus aureus DNA Aptamer by Enzyme-Linked Aptamer Assay and Isothermal Titration Calorimetry. J. Molecular Recognition, DOI 10.1002/jmr.2583, 2016.

Cagla Sonmez, Evrim Elçin, Dilan Akın, Huseyin Avni Oktem, Meral Yucel. Evaluation of novel thermo-resistant Micractinium and Scenedesmus sp. for efficient biomass and lipid production under different temperature and nutrient regimes. Bioresource Technology, 211: 422-428, 2016.

V.Cengiz Özalp, Dilek Çam, Frank J., Hernandez, Luiza I. Hernandez, Thomas Schäfer and Hüseyin A. Öktem. Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes. Analyst, 141, 2595 – 2599, DOI: 10.1039/C6AN00273K, 2016.

Frank J. Hernandez, Luiza I. Hernandez, Murat Kavruk, Yakup M. Arıca, Gülay Bayramoğlu, Barış A. Borsa, Hüseyin A. Öktem, Thomas Schäfer and Veli C. Özalp. NanoKeepers: novel stimuli responsive nanocapsules for programmed specific targeting and drug delivery. Chem. Commun., 50 (67), 9489 – 9492, 2014.

Onay M., Sonmez C, Öktem H.A., Yücel M., Thermo-resistant green microalgae for effective biodiesel production: Isolation and characterization of unialgal species from geothermal flora of Central Anatolia. Bioresource Technology, 169: 62-71, 2014.

Kalemtaş G., Chan, M., Yücel M., Öktem H.A. Evaluation of abiotic stress tolerance and physiological characteristics of potato (Solanum tuberosum L. cv. Kennebec) heterologously expressing rice Osmyb4 gene. Plant Biotechnology Reports, 8(3): 295-304, 2014.

V. Cengiz Ozalp, Gulay Bayramoglu, M. Yakup Arica, H. Avni Oktem. Design of a core–shell type immuno-magnetic separation system and multiplex PCR for rapid detection of pathogens from food samples. Appl. Microbiol. Biotechnol., DOI 10.1007/s00253-013-5231, 2013.

Murat Kavruk, Veli Cengiz Özalp, Hüseyin Avni Öktem. Portable Bioactive Paper-based Sensor for Quantification of Pesticides. J. Analytical Methods in Chemistry, Volume 2013, Article ID 932946, 1-8, http://dx.doi.org/10.1155/2013/932946, 2013.

Veli C. Özalp, Uğur S. Zeydanlı, Anita Lunding, Murat Kavruk, M. Tufan Öz, Füsun Eyidogan, Lars F. Olse, Hüseyin Öktem, Nanoparticle embedded enzymes for improved lateral flow sensors, Analyst, 138 (15): 4255-4259, 2013.

Sena Cansız, Can Özen, Ceren Bayrac ̧ A. Tahir Bayrac ̧ Fatma Gul, Murat Kavruk, Remziye Yılmaz, Füsun Eyidogan, Huseyin Avni Öktem . A sandwich-type DNA array platform for detection of GM targets in multiplex assay Eur Food Res Technol , 235:429–437, 2012.

Huseyin Avni Oktem, Ozge Senyurt, Fusun Inci Eyidogan, Ceren Bayrac, Remziye Yilmaz, Development of a laccase based paper biosensor for the detection of phenolic compounds. Journal of Food, Agriculture & Environment Vol.10 (2) 2012

Bayrac AT, Sefah K, Parekh P, Bayrac C, Oktem H.A., Tan W, In vitro Selection of DNA Aptamers to Glioblastoma Multiforme, ACS Chemical Neuroscience, 2 (3), 175-81. 2011.

Oz MT, Yilmaz R, Eyidogan F, de Graaff L, Yucel M, Oktem HA, Microarray Analysis of Late Response to Boron Toxicity in Barley (Hordeum vulgare L.) Leaves, Turkish Journal of Agriculture and Forestry, 33: 191-202, 2009.

Akcay UC, Mahmoudian M, Kamci H, Yucel M, Oktem HA, Agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik) Plant Cell Reports 28: 407-417, 2009.

Oktem HA, Eyidooan F, Demirba D, Bayrac AT, Oz MT, Ozgur E, Selcuk F, Yucel M, Antioxidant responses of lentil to cold and drought stress Journal of Plant Biochemistry and Biotechnology 17: 15-21, 2008.

Öktem H.A., Bayramoğlu G., Özalp V.C., Arica M.Y. Single step purification of recombinant Thermus aquaticus DNA-polymerase using DNA-aptamer immobilized novel affinity magnetic beads. Biotechnology Progress, 23:146-154, 2007.

Eskiocak U, Ozkan-Ariksoysal D, Ozsoz M, Oktem HA, Label-free detection of telomerase activity using guanine electrochemical oxidation signal Analytical Chemistry 79: 8807-8811, 2007.

Karabal E., Yücel M., Öktem H.A. “Antioxidant Responses of Resistant and Sensitive Barley Cultivars to Boron Toxicity” Plant Science, 164:925-933, 2003.

Mahboobi H., Yücel M., Öktem H.A. “Nitrate Reductase and Glutamate Dehydrogenase Activities of Resistant And Sensitive Cultivars of Wheat and Barley Under Boron Toxicity” J. Plant Nutrition, Vol 25 (8), 1829-1837, 2002.

Graduate program affiliation: Executive committee member of Biochemistry and Biotechnology Graduate programs of Institute of Natural and Applied Sciences; Graduate committee member of Department of Biological Sciences