Middle East Technical University Üniversiteler Mahallesi, Dumlupınar Bulvarı No:1 Biyolojik Bilimler, 244 06800 Çankaya Ankara/TURKEY

Office Room: 244

Tel: 0090 312 210 5181

Web: www.metu.edu.tr

Education: PhD: 2011, Harvard University BS: 2005, Bilkent University

Research Interest: Epigenetic control of mouse embryonic stem cell differentiation, Cell fate decisions through complex gene expression regulation, Noncoding RNAs and chromatin

Description of Research Activities: Recent work in the embryonic stem cell (ESC) field has focused on the transcriptional and epigenetic control of mESC pluripotency and self-renewal. However, it is not yet clear how a mESC decides on a certain cell fate and differentiates to a specific embryonic lineage. Prior studies showed that mESC chromatin landscape is kept in a relatively open conformation, signifying the potential for various cell fates. However, lineage specific genes should be truthfully activated at the right time and location during early embryonic development. Our lab is interested in the chromatin factors that play critical roles in the transformation of pluripotent mESCs toward restricted cell types. In an effort to understand how chromatin influences the commitment of ES cells to endodermal and mesodermal lineages, we used pooled shRNA screening and CRISPR-mediated genomic editing and identified many novel factors that regulate the kinetics or efficiency of differentiation. Ongoing experiments aim at characterizing the mechanism of action for top hits.

Selected Publications: 1. H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway N Terzi, LS Churchman, L Vasiljeva, J Weissman, S Buratowski Molecular and cellular biology 31 (17), 3569-3583 2. Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin L Vasiljeva, M Kim, N Terzi, LM Soares, S Buratowski Molecular cell 29 (3), 313-323 3. An embryonic poly (A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos E Seli, MD Lalioti, SM Flaherty, D Sakkas, N Terzi, JA Steitz Proceedings of the National Academy of Sciences of the United States of America

Graduate program affiliation: