FACULTY OF ARTS AND SCIENCESDEPARTMENT OF BIOLOGICAL SCIENCES: BIOLOGY/MOLECULAR BIOLOGY & GENETICS


Faculty

Prof. Dr. Orhan ADALI

Middle East Technical University, Department of Biological Sciences, 06800 Ankara/TURKEY

Office Room: 142

Tel: +90 312 2105182

Fax: +90 312 210 7976

E - Mail: bioorhan[-at-]metu.edu.tr

Web:

Education: B.Sc. Department of Biological Sciences, M.E.T.U.; M.Sc. and Ph.D. Department of Biochemistry, M.E.T.U.


Research Interest: Biochemistry and Molecular Biology, Biochemical Pharmacology and Toxicology, Pharmacogenetic.


Description of research activities: The characterization of the microsomal enzyme systems metabolizing drugs, environmental chemicals and carcinogens; The clarification of the possible role of flavin monooxygenases in structure and function by employing molecular biology and genetic techniques; Cytochrome P450 (CYP) and flavin monooxygenase (FMO) specific reactions; Identification of proteins in different species and tissues by using antibodies; Genotyping and phenotyping of CYP and FMO isoenzymes, paraoxanase, TPMT in human with different disorders; diabetes, stroke and cancer (leukemia); Drug metabolism and disease relation. Pharmacogenetic.


Selected Publications:

Aysun Türkanoğlu Özçelik, Birsen Can Demirdöğen, Şeref Demirkaya, Orhan Adalı “Association of Cytochrome P4502E1 and NAD(P)H:quinone oxidoreductase 1 genetic polymorphisms with susceptibility to ischemic stroke: A case-control study in Turkish population” Neurological Sciences, Vol. 38, No: 6, 1077-1085, Springer-Verlag, (DOI: 10.1007/s10072-017-2930-9), 2017.

A. Semiz, G. Çelik-Turgut, S. Karakurt, H. Akça, Ş. Arslan, O. Adalı, A. Şen “In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes” Archives of Biological Sciences, Vol. 69, No. 1, 103-110, (DOI:10.2298/ABS160208083S), 2017.

S. Karakurt, O. Adalı “Tannic Acid Inhibited Proliferation, Metastasis, Invasion of Prostate Cancer and Modulate Drug Metabolizing Cytochrome P450 and Antioxidant Enzymes” Anti-Cancer Agents in Medicinal Chemistry, Vol. 16, No. 6, 781-789, (DOI: 10.2174/1871520616666151111115809), 2016.

S. Karakurt, A. Semiz, G. Celik, A. M. Gencler-Ozkan, A. Sen, O. Adalı “Contribution of Ellagic Acid on the Antioxidant Potential of Medicinal Plant Epilobium hirsutum" Nutrition and Cancer, 68 (1), 173-183, (doi:10.1080/01635581.2016.1115092 - Taylor and Francis) 2016.

Gurbet Çelik, Aslı Semiz, Serdar Karakurt, Ayse Mine Gençler-Özkan, Sevki Arslan, Orhan Adalı, Alaattin Sen “Inhibitory action of Epilobium hirsutum extract and its constituent ellagic acid on drug-metabolizing enzymes” European Journal of Drug Metabolism and Pharmacokinetics, 41 (No: 2, April), 109-116, (DOI 10.1007/s13318-014-0238-1) 2016.

Serdar Karakurt, Melike Sever, Hasan Ufuk Çelebioğlu, Orhan Adalı “Alteration of enzyme activities and kinetic properties of GST and NQO1 with naturally occurring phenolic compounds” Turkish Journal of Biochemistry, 40(3), 251-257, 2015.

Aysun Türkanoğlu Özçelik, Birsen Can Demirdöğen, Şeref Demirkaya, Orhan ADALI “Importance of NOS3 genetic polymorphisms in the risk of development of ischemic stroke in Turkish population” Genetic Testing and Molecular Biomarkers, 18(12): 797-803, 2014.

A. Türkanoğlu Özçelik, B. C. Demirdöğen, Ş. Demirkaya, O. ADALI “Flavin containing monooxygenase 3 genetic polymorphisms Glu158Lys and Glu308Gly and their relation to ischemic stroke” Gene, 521(1-5), 116–121, 2013.

B. C. Demirdöğen, A.Ç. Adalı, Ş. Demirkaya, S. Bek and O. ADALI “Cytochrome P4501A1 genotypes and smoking- and hypertension-related ischemic stroke risk” Human and Experimental Toxicology, 32(5), 483-491, 2013.

S. Karakurt, A. Semiz, G. Celik, A. M. Gencler-Ozkan, A. Sen, O. ADALI “Epilobium hirsutum alters xenobiotic metabolizing CYP1A1, CYP2E1, NQO1 and GPx activities, mRNA and protein levels in rats” Pharmaceutical Biology, 51(5), 650-658, 2013.

Çiğdem Gökçek-Saraç, Orhan ADALI, Ewa Jakubowska-Doğru “Hippocampal levels of ChAT, PKA, phospho-PKA and phospho-CaMKIIα but not CaMKIIα positively correlate with spatial learning skills in rats” Neuroscience Letters, 545(6), 112-116, 2013.


Gurbet Celik, Asli Semiz, Serdar Karakurt, Sevki Arslan, Orhan ADALI, Alaattin Sen “A Comparative Study for the Evaluation of Two Doses of Ellagic Acid on Hepatic Drug Metabolizing and Antioxidant Enzymes in the Rat” BioMed Research International (Journal of Biomedicine and Biotechnology), Vol. 2013 (7), Article ID 358945, 9 pages, (doi:10.1155/2013/358945), 2013.

Ç. G. Saraç, S. Karakurt, O. ADALI, E.J. Doğru “Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats” Behavioural Brain Research, 235 (12), 326-333, 2012.

B. C. Demirdögen, E. Şahin, A. Türkanoğlu Özçelik, S. Bek, S. Demirkaya, O. ADALI “Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke” Molecular Biology Reports, 39 (12), 10459-10468, 2012.

A. Türkanoğlu, B. C. Demirdöğen, Ş. Demirkaya, S. Bek and O. ADALI “Association Analysis of GSTT1, GSTM1 Genotype Polymorphisms and Serum Total GST Activity with Ischemic Stroke Risk”. Neurological Sciences, 31 (6), 727-734, 2010.

B. C. Demirdöğen, Ş. Demirkaya, A. Türkanoğlu, S. Bek, E. Arınç, O. ADALI “Analysis of paraoxonase 1 (PON1) genetic polymorphisms and activities as risk factors for ischemic stroke in Turkish population”, Cell Biochemistry and Function, 27(7), 558-567, 2009.

B. C. Demirdögen, A. Türkanoglu, S. Bek, Y. Sanisoğlu, S. Demirkaya, O. Vural, E. Arinc, O. ADALI “Paraoxonase / arylesterase ratio, PON1 192Q/R polymorphism and PON1 status are associated with increased risk of ischemic stroke”, Clinical Biochemistry, 41(1-2), 1-9, 2008.

G. Ulusoy, O. ADALI, T. Boyuneğmez Tümer, G. Sahin, S. Gözdaşoğlu, E. Arinç “Significance of genetic polymorphisms at multiple loci of CYP2E1 in the risk of development of childhood acute lymphoblastic leukemia” Oncology, 72(1-2), 125-131, 2007.

T. Boyuneğmez Tümer, G. Ulusoy, O. ADALI, G. Şahin, S. Gözdaşoğlu, E. Arınç “The low frequency of defective TPMT alleles in Turkish Population: A study on pediatric patients with acute lymphoblastic leukemia” American Journal of Hematology, 82(10), 906-910, 2007.

G. Ulusoy, E. Arınç, O.ADALI “Genotype and allele frequencies of polymorphic CYP2E1 in the Turkish Population”. Archives of Toxicology, 81(10), 711-718, 2007.

E. Arınç, Ş. Arslan, A. Bozcaarmutlu, O. ADALI “Effects of diabetes on rabbit kidney and lung CYP2E1 and CYP2B4 expression and drug metabolism and potentiation of carcinogenic activity of N-nitrosodimethylamine in kidney and lung” Food and Chemical Toxicology, 45(1), 107-118, 2007.

B. C. Demirdöğen and O. ADALI “Characterization and modulation by drugs of sheep liver microsomal flavin-monooxygenase activity”. Cell Biochemistry and Function, 23, 245-251, 2005.

E. Arınç, Ş. Arslan, O. ADALI “Differential effects of diabetes on CYP2E1 and CYP2B4 proteins and associated drug metabolizing enzyme activities in rabbit liver” Archives of Toxicology, 79, 427-433, 2005.

E. Arinç, O. ADALI, A. M. Gençler-Özkan ”Induction of N-nitrosodimethylamine metabolism in liver and lung by in vivo pyridine treatment of rabbits”. Archives of Toxicology, 74(6), 329-334, 2000.

O. ADALI, G. C. Carver, R. M. Philpot “Modulation of human flavin-containing monooxygenase 3 activity by tricyclic antidepressants and other agents: Importance of residue 428” Arch. Biochem. Biophys. 358, 92-97, 1998.

J. R. Halpert, T. L. Domanski, O. ADALI, C. P. Biagini, J. Cosme, E. A. Dierks, E. F. Johnson, J. P. Jones, P. O. de Montellano, R. M. Philpot, O. Sibbesen, W. K. Wyatt, Z. Zheng “Structure-Function of cytochromes P450 and flavin-containing monooxygenases: Implications for drug metabolism” Drug Metab. Disposition, 26, 1223-1231, 1998.

O. ADALI, T. Baran, M. Y. Arıca “Characterisation of ß-galactosidase immobilised into poly (hydroxyethylmethacrylate) membranes” Biochemical Archives 14, 123-129, 1998.

O. ADALI, T. AbuBaker, E. Arınç “Immunochemical and sub-structural characterization of sheep lung cytochrome P450LgM2” Int. J. Biochem. Cell Biol. 28, 363-372, 1996.

E. Arınç , O. ADALI, R. P. K. Pasha and N. Başaran “Different influences of two fractions of lung cytochrome b5 on reconstituted lung benzphetamine N-demethylase system.” Int. J. Biochem. Cell Biol., 27, 1095-1105, 1995.

E. Arınç , O. ADALI, M. İşcan and T. Güray "Stimulatory effects of benzene on rabbit liver and kidney microsomal cytochrome P-450 dependent drug metabolizing enzymes". Arch. of Toxicol., Vol. 65, pp. 186-190, 1991.

O. ADALI and E. Arınç "Electrophoretic, spectral, catalytic and immunochemical properties of highly purified cytochrome P-450 from sheep lung". Int. J. Biochem., Vol. 22, pp. 1433-1444, 1990.


Graduate program affiliation: Biochemistry, Biology, Molecular Biology, Molecular Genetic, Biotechnology